Genetic relationship and diversity in Indian coconut accessions based on RAPD markers

Abstract

Randomly amplified polymorphic DNA (RAPD) markers were used to analyze genetic diversity and genetic relationship among coconut accessions. DNA from 81 palms representing 20 accessions, 15 Indian and 5 exotic, was used to amplify with 8 highly polymorphic primers. The 8 primers yielded 77 markers, with an average of9.6 markers per primer. The within-accession genetic diversity ranged from 0.057 to 0.196. In general, tall accessions were more heterozygous as they had higher proportions of polymorphic bands and genetic diversity. The proportion of variation explained by within accession and between accession diversity was 0.58 and 0.42, respectively. Similarly exotic accessions exhibited more variation. Dwarfs from geographically distant regions did not cluster separately. Based on the similarity matrix, cluster and principal coordinate analysis was performed. A dendrogram of genetic relationship was obtained. The extent of genetic diversity and genetic relationship among accessions is qiscussed.