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Title: | PLANT REGENERATION FROM TISSUE CULTURES OF PIPER COLUBRINUM Link |
Authors: | NIRMAL BABU, K REMA, J ZACHARIAH, T JOHN SAMSUDEEN, K RAVINDRAN, P N |
Keywords: | black pepper Piper Colubrinum Link |
Issue Date: | 1996 |
Citation: | Journal of Plantation Crops, 1996, Vol.24(Supplement), pp.594-596 |
Abstract: | Piper Colubrinum Link is a distant relative of the cultivated black pepper (Piper nigrum L. ) and is species reported to be totally immune to Phytophthora foot rot of black pepper, This species can be successfully micropropagated from ,leaf, stem and root tissues on WPM as a basal medium supplemented with growth regulators. Shoot tip explants developed excellent root system on hormone free medium and they developed multiple shoots on woody plant rnedium (WPM) supplemented with cytokinin. plantlets can be regenerated from stem, leaf and root tissues by direct organogenesis as well as through callus phase on WPM + 3 mgl¯ˡ benzyladenine (BA) and 1 mgl¯ˡ Kinetin. This tissue and callus culture protocols may help in attaining the ultimate objective of transfer of resistance from P. colubrinum to cultivated black pepper through genetic engineering. |
URI: | http://hdl.handle.net/123456789/903 |
Appears in Collections: | CROP IMPROVEMENT |
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REPRINT-065.pdf | 159.2 kB | Adobe PDF | ![]() View/Open |
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