In vitro plant regeneration from leaf-derived callus in ginger (Zingiber officinale Rosc.)

Abstract

Excised tissues from young leaves of ginger C.V. Maran were cultured on revised Murashige and Skoog medium supplemented with variolus concentrations of growth regulators. The presence of 2, 4-D in the culture medium at 9,0-22,6 µM resulted in callus growth, Organogcnesis and plantlet formation occurred when the concentration of 2,4-D is reduced to lI,9 fLM and with the addition of 44.4 fLM BA into the medium. The rate of plant regeneration increased when the growth regulators arc completely removed from the culture medium in the subsequent subcultures. The plantlets developed extensive root systems when they were put in MS liquid medium with 5,4 µM of NAA, The establishment of these plantlets in soil is about 80%.