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http://localhost:8080/xmlui/handle/123456789/834| Title: | Method for isolation of soil DNA and PCR based detection of ginger wilt pathogen, Ralstonia solanacearum |
| Authors: | Kumar, A ANANDARAJ, M |
| Keywords: | PCR specific primers Ralstonia solanacearum ginger |
| Issue Date: | 2006 |
| Citation: | Indian Phytopath., Vol.59 (2), (2006), pp.154-160 |
| Abstract: | An efficient DNA isolation protocol and PCR based detection of bacterial pathogen in soil are described here. The use of this DNA isolation protocol and PCR based method using universal Ralstonia solanacearum specific primer offer a rapid method for unambiguous detection of this pathogen in soil which can be employed for monitoring soil. The PCR based assay could detect the pathogen at a concentration of 103-104cells per gram of soil. |
| URI: | http://hdl.handle.net/123456789/834 |
| Appears in Collections: | CROP PROTECTION |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Method for isolation of soil DNA and PCR based detection.pdf | 217.12 kB | Adobe PDF |  View/Open |
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