Please use this identifier to cite or link to this item:
http://localhost:8080/xmlui/handle/123456789/610
Title: | Isolation and Amplification of Genomic DNA from Recalcitrant Dried Berries of Black Pepper (Piper nigrum L.)—A Medicinal Spice |
Authors: | Dhanya, K Kizhakkayil, Jaleel Syamkumar, S Sasikumar, B |
Keywords: | Black pepper DNA isolation PEG Geographical Indication Molecular markers |
Issue Date: | 2007 |
Citation: | Mol Biotechnol, 2007 Oct;37(2):165-168. |
Abstract: | Black pepper is an important medicinal spice traded internationally. The extraction of high quality genomic DNA for PCR amplification from dried black pepper is challenging because of the presence of the exceptionally large amount of oxidized polyphenolic compounds, polysaccharides and other secondary metabolites. Here we report a modified hexadecyl trimethyl ammonium bromide (CTAB) protocol by incorporating potassium acetate and a final PEG precipitation step to isolate PCR amplifiable genomic DNA from dried and powdered berries of black pepper. The protocol has trade implication as it will help in the PCR characterization of traded black peppers from different countries. |
URI: | http://hdl.handle.net/123456789/610 |
Appears in Collections: | CROP IMPROVEMENT |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.