Evaluation of genetic stability of micropropagated plants of three species of Garcinia using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers

Abstract

Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to analyze the genetic stability of micropropagated as well as mother plants of three species of Garcinia, viz., G. gummigutta, G. indica, and G. tinctoria. Twelve RAPD primers and four ISSR primers generated clear, distinct and reproducible bands. Total genomic DNA extracted from fresh leaves of in vitro regenerated as well as mother plants was used for developing RAPD and ISSR profiles. All RAPD and ISSR profiles from micropropagated plants were monomorphic and similar to those of the field grown mother plants. No genetic variation was detected within the micropropagated plants in comparison to their mother plants. The results indicate that the Garcinia plants regenerated using micropropagation system standardized at our laboratory were genetically stable.