Random amplified polymorphic DNA technique for detection of plant based adulterants in chilli powder (Capsicum annuum).

Abstract

The present study reports a modified protocol for isolation of good quality DNA from chilli powder and its probable adulterants (dried red beet pulp, almond shell dust and powdered Ziziphus nummularia fruits) and the utility of RAPD primers for detection of adulterants in marketed chilli powder. Selected RAPD primers, which produced adulterant specific bands in simulated samples were used for analyzing market samples of chilli powder. Out of the six market samples analyzed, one sample amplified Z. nummularia specific band indicating the occurrence of adulteration in market samples. All the market samples tested were free from dried red beet pulp or almond dust adulteration.