Extracellular Myco-synthesis of Silver Nanoparticles from and Trichoderma virens Metarhizzium anisopliae

Abstract

Application of nanotechnology in the field of agriculture enhance the efficacy of input use to increase the yield. [n the present investigation a total of six isolates of TrichodermG virells and two isolates of Metarhizzillm Gnisopliae were used to check its reducing properties of silver into nanosilver particle. All the isolates produced silver nano particle but the intensity varied, among the tested isolates. The isolate, T virells (T4) could produce maximum nanopartides as evident from the UV-Vis study. The absorbance was recorded at 420 nm at every 24 h interval and maximum intensity was noticed at fifth day in T virells (T4) (0.142) followed by Melarhizill/ll ITCC 5414 (0.120) but on first day. Further, the high resolution transmission electron microscopy (HRTEM) provided the shape and size of nanopartilce synthesized. The nanoparticls were found single and also as aggregated fonn with more or less uniform in shape and size of8-60 nm. Thus the result confinns the production of silver nanoparticles through reduction by extracellular culture filtrate.