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DC Field | Value | Language |
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dc.contributor.author | Sasi, Shina | - |
dc.contributor.author | Revathy, K A | - |
dc.contributor.author | Bhat, A I | - |
dc.date.accessioned | 2018-11-08T09:01:35Z | - |
dc.date.available | 2018-11-08T09:01:35Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | J. Plant Biochem. Biotechnol. (October–December 2015) 24(4):466–469 | en_US |
dc.identifier.uri | http://hdl.handle.net/123456789/1807 | - |
dc.description.abstract | A loop-mediated isothermal amplificationb(LAMP) and real-time LAMP based assays were developed for quick and sensitive detection of transgenic black pepper plants. Primers (six each) were designed based on the nucleotide sequence of two target regions [kanamycin and Cauliflower mosaic virus (CaMV) 35S promoter] integrated into the genome of transgenic black pepper. Both assays successfully detected the transgenic plants and no cross-reaction was recorded with non-transgenic plants. The sensitivity of LAMP was up to 104 times that of conventional PCR while real-time LAMP was up to 103 times that of LAMP and 107 times to that of PCR. The addition of 6 mM magnesium sulphate and 0.4 M betaine with 1 h reaction time proved optimal for amplification through LAMP assay. The assays were validated by testing putative transformants of black pepper. The present study clearly established that LAMP and real-time LAMP assays can provide a rapid and simple approach for screening transgenic black pepper and other plants transformed by using the above target gene sequences. | en_US |
dc.language.iso | en | en_US |
dc.subject | CaMV35S promoter | en_US |
dc.subject | Detection | en_US |
dc.subject | Kanamycin | en_US |
dc.subject | Sensitivity | en_US |
dc.subject | Validation | en_US |
dc.title | Rapid identification of transgenic black pepper using loop-mediated isothermal amplification (LAMP) and real-time LAMP assays | en_US |
dc.type | Article | en_US |
Appears in Collections: | CROP PROTECTION |
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File | Description | Size | Format | |
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261 Sasi2015_Article_RapidIdentificationOfTransgeni.pdf | 342.95 kB | Adobe PDF | View/Open |
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