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Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/1636
Title: Kanamycin sensitivity of cultured tissues of Piper nigrum L.
Authors: Sasikumar, B
Veluthambi, K
Keywords: Agrobacterium
black pepper
callus
kanamycin sensitivity
Piper nigrum
Issue Date: 1994
Citation: Journal of Spices & Aromatic Crops, Vol.3, No.2, pp.158-160, 1994
Abstract: The kanamycin sensitivity for callus growth was studied in vitro in a cultivar of black pepper (Piper nigrum) using cotyledons as explants to investigate the suitability of kanamycin resistance as a selectable marker for Agrobacterium mediated transformation. Callus formation was completely inhibited at 50 µg ml¯ˡ and above concentrations of kanamycin suggesting that 50 µg ml¯ˡ is the minimum concentration needed to select the transformed tissues.
URI: http://hdl.handle.net/123456789/1636
Appears in Collections:CROP IMPROVEMENT

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