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Title: | Kanamycin sensitivity of cultured tissues of Piper nigrum L. |
Authors: | Sasikumar, B Veluthambi, K |
Keywords: | Agrobacterium black pepper callus kanamycin sensitivity Piper nigrum |
Issue Date: | 1994 |
Citation: | Journal of Spices & Aromatic Crops, Vol.3, No.2, pp.158-160, 1994 |
Abstract: | The kanamycin sensitivity for callus growth was studied in vitro in a cultivar of black pepper (Piper nigrum) using cotyledons as explants to investigate the suitability of kanamycin resistance as a selectable marker for Agrobacterium mediated transformation. Callus formation was completely inhibited at 50 µg ml¯ˡ and above concentrations of kanamycin suggesting that 50 µg ml¯ˡ is the minimum concentration needed to select the transformed tissues. |
URI: | http://hdl.handle.net/123456789/1636 |
Appears in Collections: | CROP IMPROVEMENT |
Files in This Item:
File | Description | Size | Format | |
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JSAC-001.pdf | 2.61 MB | Adobe PDF | View/Open |
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