Cloning and Characterization of Genes Encoding Two Detoxifying Enzymes, Glutathiones Transferase and Carboxylesterase, from Burrowing Nematode (Radopholus Similis)

Abstract

Radopholus Similis (Cobb) Thome is a migratory endoparasitic nematode infesting several tropical and subtropical plant species. Computational Screening and conserved domain annotation of assembled EST sequences from the burrowing nematode (R.Similis) revealed seven contigs similar to glutathione S-Transferases (GSTs) an dfour contigs for carboxylesterases/cholinesterases (CESs). One of the contigs corresponding to each gene were doned from R.Similis cDNA (KM670018, KP027005) and characterized by phylogeny and structural motif comparison. Glutathione S-Transferases is a critical antioxidant and detoxification enzyme and carboxylesterase is responsible for controlling the nerve impulse, detoxification and various developmental functions. Detoxifying ability of these proteins makes them as major targets of pesticides used for plant parasitic nematode (PPN) management