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Title: | Endophytic interactions of Trichoderma harzianum in a tropical perennial rhizo-ecosystem |
Authors: | Umadevi, P Anandaraj, M Benjamin, Sailas |
Keywords: | Fungal endophyte Arbuscular micorhizal fungi Trichoderma harzianum Piper nigrum |
Issue Date: | Mar-2017 |
Citation: | Research Journal of Biotechnology, Vol.12, No.3, March 2017, pp.22-30 |
Abstract: | This study demonstrates the endophytic interaction of the well-known growth promoting and biocontrol agent in black pepper. Trichoderma harzianum, coupled with its rhizosphere fungal flora was evidenced from metagenomics. We employed short-term and long-term strategies to study the interactions of T. harzianum in black pepper rhizosphere. In short-term strategy, T. harzianum was co-cultivated with axenic plantlets while pot culturing of plants in soil mixed with T. harzianum was performed in the long-term strategy. The colonization was investigated by light microscopy and scanning electron microscopy (SEM). The co-cultivation of T. harzianum with black pepper showed the intercellular colonization at 24 h and formation of intracellular hyphae with vesicles at 48 h of interaction. The long term strategy inferred that T. harzianum was able to colonize the black pepper roots along with the AMF inter- and intra-cellularly. The whole genome metagenomic sequencing brings out the population abundance of the entire rhizosphere fungal flora. |
URI: | http://hdl.handle.net/123456789/1423 |
Appears in Collections: | CROP PROTECTION |
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