A method for isolation and reverse transcription of high quality RNA from Piper species

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DC Field	Value	Language
dc.contributor.author	GEORGE, K JOHNSON	-
dc.contributor.author	Sandeep Varma, R	-
dc.contributor.author	Ganga, G	-
dc.contributor.author	ANANDARAJ, M	-
dc.contributor.author	PARTHASARATHY, V A	-
dc.date.accessioned	2018-01-04T05:47:00Z	-
dc.date.available	2018-01-04T05:47:00Z	-
dc.date.issued	2005	-
dc.identifier.citation	Journal of Spices and Aromatic Crops, Vol.14, No.1, pp.10-14, 2005	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/1213	-
dc.description.abstract	A rapid and efficient protocol for isolation of RNA from Piper leaves is described. RNA was extracted from two Piper species namely, P. nigrum and P. colubrinum, using strong denaturing buffer containing guanidinum thiocyanate and polyvinylpyrollidone followed by precipitation with pre-cooled sodium acetate and extraction with phenol:chloroform:isoamylalcohol (25:24:1). RNA was precipitated with isopropanol. The extraction procedure reliably yielded high quality RNA suitable for reverse transcription experiments.	en_US
dc.subject	Piper spp.	en_US
dc.subject	Reverse transcription	en_US
dc.subject	RNA isolation	en_US
dc.title	A method for isolation and reverse transcription of high quality RNA from Piper species	en_US
dc.type	Article	en_US
Appears in Collections:	CROP IMPROVEMENT

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