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Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/1071
Title: Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies
Authors: LI NCY, A
Sasikumar, B
Keywords: Aerial stem
biological hardening
direct regeneration
ginger
primary thickening meristem
Trichoderma harzianum
Issue Date: 2010
Citation: Turk J Bot, 2010, Vol.34, pp.21-29
Abstract: A protocol for direct plantlet regeneration from aerial stem ex plants of ginger (Zingiber officinale Rosc.) has been developed. Adventitious shoots and roots were regenerated from in planta aerial stem explants of2 ginger varieties viz. var. 'Jamaica' and var. 'Varada: Maximum shoot and root regeneration were observed in the cultures containing TDZ and IBA (1: 1; I:O.ll11g L-t) in both varieties. Cultures containing TDZ alone showed poor shoot and root formation. Histological studies revealed that the shoal initials originated from the primary thickening meristem and apical meristem, while the root primordial originated from the primary thickening meristem present in the aerial stem. The hardening of in vitro plants was performed wit h different hardening media and successful acclimatization was obtained. The hardening medium containing soil: sand: coir dust: cow dung + 5 g trichoderma harzianum/cup gave good result in all aspects like survival of hardened plants, plant height, number of leaves, and chlorophyll content.
URI: http://hdl.handle.net/123456789/1071
Appears in Collections:CROP IMPROVEMENT

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